B. Nageshwari
| Designation: | Lecturer | Qualification | M.Sc., Ph.D., Univ of Hyd |
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Introduction
B.NAGESHWARI, M.Sc., Ph.D.
Lecturer
Department Of Biotechnology
Govt College (A)
Rajamahendravaram
E.G.Dist, A.P. 533105, India.
Email: b.nageshwari@gcrjy.ac.in
Positions held :
Lecturer in Department of Biotechnology Govt College (A), Rjy... (26th Dec 2011- to date)
Academic Consultant at Mahatma Gandhi University, Nalgonda District, Telangana State. ( Feb 2010 -Nov 2011)
Position in Committees at : Govt ( Autonomous ) College, Rajamahendravaram
Assistant Nodal Officer - Food Processing and Preservation course (2015-17)
Mentor & Coordinator, Centre for Innovation Incubation and Entrepreneurship (2016 to date)
Monitoring Committee Member, Community College (2105 to date)
Coordinator : Medical Inspection Committee ( 2017 - to date )
Coordinator -Auction committee -(2017-to date)
Committee Member, U.G.C. (2103 to date)
Committee Member, Value Education Cell (2013 to date)
Committee Member, Green Cell (2016 - to date)
Committee Member Red Ribbon Club and Red Cross (2106- to date)
Medical Inspection Committee Member (2013 to 2016)
Admission Committee Member -Biotechnology-Govt College (A), Rjy (2013 to date)
Green Audit Committee Member –2017--18
Community College--Admission Committee Member --2015-17
Annual Stock Verification ---Committee Member---- 2013-17.
Community College---Staff Selection Committee Member -- 2015-16.
Anti Ragging Squad on Campus 2015-16
Women Empowerment Cell--Committee Member----2014-16.
Soft launch of Digital Classrooms --Committee Member---2017
Student interaction committee member.
Member : Advisory board : DST FIST –Project Implementation
Securitizing committee : for appointment of FDP substitute teacher in Biotechnology.
Chairperon, UG Board of Studies in Biotechnology, Adikavi Nannaya University Rajamahendravaram (2013- to 2017 )
Education
M.Sc. Biotechnology (2001) : Pune Univerity, Pune, Maharashtra, India.
Ph.D. Life Sciences (2010) : Hyderabad Central University, Hyderabad, Telangana State, India
Research Interests:Cancer Biology, Immunology , Bioremediation of waste water and Biohydrogen production
Specialization : Cancer Biology and Immunology.
Areas of Research
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Dr.B.NAGESHWARI
Research:- Papers & Reviews & Articles.
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S.No
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Title of the Paper
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Year
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Journal Details
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ISSN No
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Impact factor
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| 21. |
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| 20 |
PATENT
Novel Methodologies for early weed detection for improvising crop yield using the algorithms of machine learning.
(PDF)
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Friday
30-12-2022
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Official Journal Of Patent Office.
Issue No 52/2022
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| 19 |
Targeting the TIMPs with PROTAC-based small molecules as a potential
(PDF)
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13th Apr 2021 |
TCABSE-J Applications report
TCABSE-J, Vol. 1, Issue 0, Apr 13th 2021
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| 18 |
Effect of structural flexibility in HIV-1 gp-41 on its antigenic properties thatchallenge the antibody engineering for passive immunization therapy.
( PDF )
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25 Oct 2020 |
TCABSE-J Research Article |
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| 17 |
Mapping and analysis of cathepsin cleavage site distribution on the 3-D modelof human thyroglobulin for potential inhibitor design.
(PDF)
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25 Oct 2020 |
TCABSE-J Analysis Report
TCABSE-J inaugural edition, Oct 25th 2020
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| 16 |
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Leveraging the active site steric hindrance for GTP hydrolysis in mutant
K-Ras variants to achieve selectivity in kinase inhibitors for cervical cancer
(PDF)
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25 Oct 2020 |
TCABSE-J Applications report
TCABSE-J inaugural edition, Oct 25th 2020
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| 15 |
In silico mutagenesis and molecular modeling analysis of PTEN protein to
(PDF)
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25 Oct 2020 |
TCABSE-J Communications
TCABSE-J inaugural edition, Oct 25
th
, 2020.
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14.
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Vantage point: New insights into the old enteric fever treatment by rescuing
the host cell ubiquitin-proteasome system.
(PDF)
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Oct 25
th
, 2020.
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TCABSE-J Communications
Oct 25th , 2020.
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13
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EST-SSRA and(GATA)n Motif specific SSR Markers For Geneticdiversity Analysis and Identification of Genotype By Marker Combinations in Basmati accessions
( PDF )
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31 Mar
2019
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Asian Journal of Biochemical and Pharmaceutical Research
Issue 1
(Vol. 9)
2019
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ISSN:
2231-2560
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0.96
5 Years Impact Factor: 2.64
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12
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Alkaline Phosphatase Activity of Normal and Malignant Human Lymphocytes
( PDF )
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Jul
2019
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Indian J Clin Biochem.
34 (3)
272 - 279
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ISSN
0970-1915
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11
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Toddy mediated synthesis of Ag-Ni bimetallic nanoparticles and their biological evaluation
( PDF )
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Dec
2018
-
Feb
2019
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International Journal of Green and Herbal Chemistry
IJGHC,
Sec. A; Vol.8, No.1, Pg 099-108.
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E-ISSN:
2278-3229
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2.056
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10
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Studies on 3H-levamisole binding to murine splenic lymphocytes, normal, malignant human
lymphocytes and fate of levamsisole in cell culture.
( PDF )
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2018
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Biomedical and Biotechnology Research Journal
Volume 2 Issue 1
53-58
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ISSN –
Print: 2588-9834,
Online: 2588-9842
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9
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Effect of levamisole on expression of CD138 and interleukin-6 in human multiple myeloma cell lines
(PDF )
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2017
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Indian J Cancer
54 (3)
566-571
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Online ISSN : 1998-4774
Print ISSN 0019-509X
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1.224
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8
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Effect of levamisole on alkaline phosphatase activity and immunoglobulin secretion of lipopolysaccharide stimulated murine splenic lymphocytes.
( PDF )
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2017
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Biomedical and Biotechnology Research Journal
1
151-156
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ISSN
ISSN- Print:
2588-9834,
Online: 2588-9842
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7
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Bioremediation of waste waters by the anoxygenic photosynthetic bacterium Rhodobacter sphaeroides SMR 009.
( PDF )
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2014
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International Journal
of Research in Environmental Science and Technology.
4(1) 16-19.
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ISSN
2249-9695
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6
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Biofabrication and Characterization of Zinc Nanorods from Aloe vera.
( PDF )
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2013
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International Journal of Science and Research‚ 2013.
148-150
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ISSN
2319-7064
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7.803
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5
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Human Alkaline phosphatases in health and disease: A mini review
(PDF )
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2013
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International Journal of Research in pharmaceutical Sciences.
4(3)
371-379
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ISSN
0975-7538
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0.604
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4
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Factors influencing the production of hydrogen by the purple non sulphur phototrophic bacterium Rhodopseudomonas acidophila KU003
(PDF )
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2012
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Microbial Biotechnology
5(6)
674-678
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ISSN
1751-7915
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5.813
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3
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Photoproduction of hydrogen under different cultural conditions by alginate immobilized Rhodopsedomonas palustris KU003.
(PDF )
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2012
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International Journal of Environment and Bioenergy
4 (3)
141 – 146
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ISSN :
2165- 8951
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2
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Influence on different cultural conditions on photoproduction of hydrogen by Rhodopseudomonas palustris KU003.
(PDF )
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2011
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International Scholarly Research Network, ISRN Renewable Energy, Volume 2011, Article ID 328984 not in scopus
1-6
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ISSN:
2090-7451
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1
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Anti-proliferative effect of levamisole on human myeloma cell lines in vitro.
(PDF )
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2010
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Journal of Immunotoxicology,
7(4)
327–332.
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ISSN
1547-6901
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4.6
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Summary of Completed Research Project
Title : Effect of levamisole on human multiple myeloma cell lines in vitro- Alkaline Phosphatase activity as a “putative” target.
Mitogen / antigen stimulated B lymphocytes express Alkaline Phosphatase (APase) activity. In resting Bcells and in resting & activated T cells, APase activity is negligible. Enhanced enzyme activity has beenobserved during the proliferative phase and differentiation of B cells ( in mice and rats ) indicating thatthe enhanced expression of APase correlates well with proliferation and differentiation of Bcells.Lymphocytes from normal human did not express APase activity, where as lymphocytes isolatedfrom bone marrow samples of multiple myeloma patients showed significantly high APaseactivity.Human Myeloma cell lines (RPMI 8226 and U266 B1) as well as bone-marrow derived myeloma cellsfrom multiple myeloma patients express APase activity. Therefore, myeloma cells (malignantdifferentiated B cells) offer an experimental system to understand the role of APase in B celldifferentiation and carcinogenesis. Levamisole, an inhibitor of TNAPase has been used to study its effectson TNAPase and associated functions. Levamisole inhibited the mitogen-induced proliferation anddifferentiation of B cells and the proliferation of the myeloma cell lines via inhibition of APase in murinesystem and cell lines in a dose-dependent manner. Levamisole also inhibited the release ofimmunoglobulin by the myeloma cells indicating a role of APase in proliferation and differentiationfunction (immunoglobulin secretion).The function of APase in dephosphorylation was monitored by expression of CD138 on the myelomacells. The shedding of CD138 has been shown to be increased by tyrosine phosphorylation of itscytoplasmic domain. APase is involved in dephosphorylation of the tyrosine. Inhibition of APase bylevamisole resulted in phosphorylation of cytoplasmic domain there by causing subsequent shedding ofCD138. The binding of levamisole to alkaline phosphatase in normal and myeloma cells has been hasbeen confirmed using 3H-levamisole.
Techniques used:
• Isolation of lymphocytes from mouse spleen, human peripheral blood and bone marrow samplesof myeloma patients.
• Culture and Maintenance of isolated primary cells and established cell lines both adherent and suspension culture.
• Cell lines handeled : B cell lines : RPMI 8226 and U66 B1, T cell line : Sup T1, Hep G2 : Yac -1: murine T cell lymphoma.
• Mitogenic / antigenic stimulation of primary lymphocytes isolated from mouse and human peripheral blood.
• Lymphocyte proliferation and cyotoxicity assays using 3H-Thymidine and MTT.
• Cytochemical staining of lymphocytes.
• Purification of Alkaline phosphatase.
• Activity staining of enzyme on polyacryamide gel.
• Phosphatase assays : alkaline phosphatase and pyrophosphatase.
• Imunostaining, Immunofluorescence, , Confocal microscopy, Immunoprecipitation, immunodiffusion.
• Raising polyclonal antibodies / Preparation of antiserum, ELISA, Blotting technic.
• Dot Blot assay
• Flow cytometry (FACS)
• Isolation of DNA, RNA and protein.
• SDS and Native PAGE gels.
• Microbial culture, Preparation of competent cells, E. coli transformation, Molecular cloning, designing primers.
• PCR, RT-PCR, Agarose gel electrophoresis.
• Phosphatase assay.
• Intracellular localisation of enzyme alkaline phosphatase using 3H-levamisole
• Assays for Apoptosis :Caspase assay, DNA fragmentation, Cytochrome - c assay.
• Chromatography: Gel exclusion, Ion-exchange, Affinity, TLC, HPLC.
Seminars And Events Participated
Paper Presentations/ Invited Talks:
• Innovation through Biotechnology is a pleasant responsibility: ( Resource person ) National Seminar : 26th Feb 2018 : SVD Government College (Women), Nidadavole, West Godavari Dist., A.P.
• Mana TV: Resource Person for Biotechnology. “ Constructionog Genomic and cDNA libraries”.
• Endocrine System – Disorders & Stress Response : ( presented paper) National Seminar : 16th& 17th Dec 2014 :Government College (Autonomous) Rajamahendravaram, A.P.
• Effect of levamisole on human multiple myeloma cell lines invitro- Alkaline phosphatase activity as a “putative” target. Presentation given at BioQuest-2008, Hyderabad CentralUniversity, Hyderabad-India.
As Organizing Committee Member
• Renewable Energy Research and Education ( Organising Committee member ) International Conference : 8th -10th Feb 2018.
• Biomass to Bioenergy ( Organising Secretary) National workshop : 19th and 20th Dec 2017 : Government College (Autonomous) Rajamahendravaram, A.P.
• Digital Science Lab (Organizing Member) Workshop: 24th Nov 2016: Govt College (A),Rajamahendravaram. A.P.
• Advances Break through, challenges and Developments in Foodomics: National Seminar (Co-Convenor) 10th and 11th Nov 2016: Govt College (Autonomous) Rajahmahendravaram. A.P.
• Data Analysis & its applications: (Organizing Committee Member) National Workshop: 30th Jan2015: Government College (Autonomous), Rajamahendravaram. A.P.
Poster presented
• Advances, Break throughs, challenges and Developments in Foodomics : “Making food safe fromfarm to plate” : National Seminar (Co-Convenor) 10th and 11th Nov 2016 : Govt College (Autonomous) Rajamahendravaram
• “Bacterial cell wall lipopolysaccharide in species specific immune stimulation”. Presented Poster at National Seminar on Microbial Diversity –Exploration and Bioprospecting 27- 28 March, 2010. Department of Microbiology, Kakatiya University, Warangal, India.
• Tissue Non-specific alkaline Phosphatase of B lymphocytes : Functional Analysis usinglevamisole as a tool”. Presented at 5th International Alkaline Phosphatase Symposium . May16-19th, 2007 Huingne –France. (Organised by Hypophosphatasia Europe).
• “Inhibition of Syndecan-1 expression and M-Protein secretion in myeloma cell Lines in vitro by Levamisole”. Presented Poster at 32nd Annual Conference of Indian Immunology Society 24-27th Nov- 2005. Post Graduate Institute of Medical Education and
Research, Chandigarh-India.
• “Anti-proliferative effect of levamisole on human myeloma cell lines-RPMI 8226 and U266B1”.Presented Poster at 30th Annual Conference of Indian Immunology Society: 23-25th Nov- 2003. Sanjay Gandhi Institute of Medical Sciences, Lucknow-India.
Attended : Conferences/Workshops/Seminars/Symposiums/Trainings/Faculty Development Programmes.
2018
Intellectual Property Rights & Its Education For Young Minds, at Govt ( A) College , Rjy. National Workshop conducted by Board of Research Studies and Academic Cell, Govt ( A) College , Rjy, on 21 April 2018
Challenges and Opportunities in Andhra Pradesh After Bifurcation, Govt( A) College , at Rjy.National Workshop conducted by Faculty of Social Sciences, Govt ( A) College , Rjy on 28th March 2018
Practical Entrepreneurship with Realtime Entrepreneurs, National Workshop , conducted by Department of Commerce: Govt ( A) College , Rjy. 14th March 2018.
Innovation through Biotechnology is a pleasant responsibility: National Seminar : 26th Feb 2018: SVD Government College (Women), Nidadavole, West Godavari Dist., A.P.
Renewable Energy Research Education: Inernational Conference: 9-10 feb 2018. GovernmentCollege (Autonomous) Rajamahendravaram.
2017
• Biomass to Bioenergy: National Workshop: 19-20 Dec 2017. Government College (Autonomous)Rajamahendravaram.(Organising Secretary)
• Cognitive Behavior Therapy and Social Skills Training: National Workshop: 15-16 December 2017.AdikaviNannaya University, Rajamahendravaram.
• Virtual Classes : Workshop : on 17th Oct 2017 : Organised by commissionerate of Collegiate education, A.P., Vijayawada. At Diechman Hall, Acharya Nagarjuna University, Guntur.
• Three Day Residential (Faculty Development) Training Programme on New Market Oriented Restructured Courses, Aquaculture Technology, Sericulture Management &Paramedical science”: 9th to 11th Aug 2017, AP HRDI, Bapatla.
• STEM Teacher Training Workshop on Research Based Pedagogical Tools . 26th Feb to 1st Mar2017.IISER, Pune, Ministry of Human Resource Development, Govt of India.
• Pedagogy and e-content development: National Workshop: 19th Jan 2017: Andhra Loyola College, Vijayawada, A.P.2016
• “Limited Liability Partnership – A New Opportunity”: National Seminar: 21-22 December 2016:Government College (Autonomous) Rajamahendravaram.
• Language skills employment opportunities: State Level Seminar: 15th Dec 2016: Government College (Autonomous) Rajamahendravaram.
• MOOCs: Workshop: 30th Nov & 1st Dec 2016: Government College (Autonomous) Rajamahendravaram.
• Digital Science Lab :Workshop:24th Nov 2016 : Govt College (A), Rajamahendravaram.Organizing Member
• Advances, Break throughs, challenges and Developments in Foodomics : National Seminar (Co-Convenor) 10th and 11th Nov 2016 :Govt College (Autonomous)Rajahmahendravaram.
• UGC sponsored one day international symposium on “Cellular Stress : International Symposium : 10th Aug 2016 :Govt College (Autonomous) Rajahmahendravaram.
• Promotion of Nature Friendly Practices in Farming for Health of Environment and Mankind : Workshop &Training : 4th & 5th mar 2016 : AdikaviNannaya University : Rajamahendravaram.
2015
• Semester System Orientation Workshop : 26th & 27th Nov 2015 :Adikavinannaya University Rajamahendravaram.
• Light Emitting Devices &Materials: Faculty Development Program cum Workshop : 20th Nov 2015: Government College (Autonomous) Rajamahendravaram.
• Right to Information Act : Training : 18th to 22nd May 2015 : Dr. MCR HRD Institute, Hyderabad
• E-Governance: Problems &Prospects : International Seminar : 8th Mar 2015: Government College (Autonomous) Rajamahendravaram
• Research Methodology: National Workshop: 7th Mar 2015 : Government College (Autonomous)Rajamahendravaram.
• Data Analysis &its applications: National Workshop: 30th Jan 2015: Government College (Autonomous) Rajamahendravaram. : Organizing Committee Member.
2014
• Endocrine System – Disorders & Stress Response National Seminar : 16th & 17th Dec 2014 :Government College (Autonomous) Rajamahendravaram: ( presented paper)
• Advanced Technology Oriented Materials, ATOM-2014 : National Conference : 8th & 9th Dec 2014 : Government College (Autonomous) Rajamahendravaram
• Radiochemistry & Applications of Radioisotopes : National Workshop : 24th to 29th Nov 2014 : IANCAS and Government College (Autonomous) Rajamahendravaram
• Stress Resilience Techniques: InternatoinalWorkshop : 16thNov 2014 : Government College (Autonomous) Rajamahendravaram
• Nano Materials – Synthesis & Characterization Techniques State Level Seminar : 28thFeb 2014 :(Presented paper) Government College (Autonomous) Rajamahendravaram.
• Teaching English Grammar at UG Level-A New Perspective.: State Level Seminar : Government College (Autonomous) Rajamahendravaram :: 26thFeb 2014:
• Awareness workshop on Telugu poetry and changing trends.: State Level Workshop : 25th Feb2014: Government College (Autonomous) Rajamahendravaram
• Critical Analysis of Hindi and Telugu Literature. National Seminar: 10th & 11th Jan 2014 : Government College (Autonomous) Rajamahendravaram.
2012
• Capacity Building of Women Managers in Higher Education : Workshop : 27th – 31st Aug,2012 ,Univ College of Technology, Osmania University
• Social Reform & Education – A: Tool of Women Empowerment-some reflections.: National Seminar: 18th& 19th Mar 2012: SreeKandukuriVeereshalingam Theistic College, Rajahmundry
• Training Programme on Environmental Governance to Combat Climate Change 16th to 18thFeb 2012, Dr. MCR HRD Institute, Hyderabad.
Till ---2011
• 6-Day Teacher Induction Training Program : Training : 26th to 31st Dec 2011 : Government College (Autonomous) Rajamahendravaram
• Microbial Diversity –Exploration and Bioprospecting : National Seminar . 27th - 28th Mar 2010. : Department of Microbiology, Kakatiya University, Warangal, Telangana State.
• Indo-German workshop on Molecular Epidemiology of Infectious Diseases- held at University ofHyderabad during November 28-30, 2008.
• 5th International Alkaline Phosphatase Symposium, : (Presented Poster) : International Symposium,: May 16th to 19th 2007 : Organised by Hypophosphatasia Europe. Huingne – France.
• International Workshop on defining the future –HIV/ AIDS-Next 25 years. : University of Hyderabad, Hyderabad –December 18-20, 2006.
• 32nd Annual Conference of Indian Immunology Society 24-27th Nov- 2005. Post Graduate Institute of Medical Education and Research, Chandigarh-India.
• 31st Annual Conference Indian Immunology Society, Nov-2004.Centre for Biotechnology, AnnaUniversity, Madras-India.
• 30th Annual Conference of Indian Immunology Society: 23-25th Nov- 2003. Sanjay Gandhi Institute of Medical Sciences, Lucknow-India.
• Continuing Medical Education course on ‘Basic Immunology’ held at Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow on November 22,2003.
Detailed CV
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